Conclusions These modifications are simple and easily implemented so that study of rare leukocyte subsets using scarce or expensive reagents can occur.Ī comparative study of the effects of cone- plate and parallel-plate geometries on rheological properties under oscillatory shear flowįull Text Available -1 Korea-Australia Rheology Journal A comparative study of the effects of cone- plate and parallel- plate geometries on rheological properties under oscillatory shear flow Hyeong Yong Song1, Reza Salehiyan2, Xiaolei Li1, Seung Hak Lee1 and Kyu Hyun1. We show that modifications in the chamber size, internal tubing diameters, injection valves, and a recirculation design may dramatically reduce the cellular and reagent requirements without altering measurements. Results In this study, we evaluate modifications to a newer parallel plate flow chamber design in comparison to the original parallel plate flow chamber described by Lawrence et al. In addition, the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. Improvements to parallel plate flow chambers to reduce reagent and cellular requirementsĭirectory of Open Access Journals (Sweden)įull Text Available Abstract Background The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. The governing partial differential equations were solved numerically using Crank-Nicolson implicit method to represent the flow behavior of the fluid One of the plates was stationary and the other plate moved with constant velocity. In this investigation an analytical solution was obtained to predict the steady and unsteady Couette flow between parallel plates. International Nuclear Information System (INIS)Ĭouette flow between parallel plates is a classical problem that has important applications in various industrial processing. Computational study of Couette flow between parallel plates for steady and unsteady cases
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